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Oilseed rape (Brassica napus) and vegetable Brassicas (Brassica oleracea)

Brassica The Brassicaceae family consists of many important field crops and vegetables such as oilseed rape, the Brassica oleracea vegetable group (cauliflower, broccoli, brussels sprouts, kale) as well as other species such as Brassica rapa and radish (Raphanus sativus).


Microsatellite markers

Over the years, TraitGenetics has developed a set of more than 1500 high quality microsatellite markers for oilseed rape that have also been tested on related Brassica species.

The functionality of molecular markers in related species depends upon their genetic distance. Our molecular markers originating from rapeseed are frequently also functional in the ancestors of rapeseed B. napus, B. rapa and B. oleracea respectively and to a lesser extent also in more distantly related species such as radish.


Fragment analysis of Brassica napus microsatellite markers for functionality and polymorphisms in Brassica vegetable species.


SNP markers

For the identification of SNP markers in oilseed rape and other Brassica species, we have so far sequenced approximately 3500 amplicons derived from Brassica ESTs. This panel is designed to represent the entire range of genetic diversity in the allopolyploid oilseed rape species. Through the additional analysis of DNA pools from Brassica oleracea and Brassica rapa, we can assign detected SNPs to either one of the diploid parental genomes.

SNP marker identification in B. napus and B. rapa

A molecular marker database for Brassica napus

200 mapped SSR markers were selected according to their suitability for genotyping and used to analyse more than 1000 current rapeseed varieties, older varieties and important germplasm. Our data base today consists of more than 342.000 entries (alleles). The high quality data were used to analyze genetic relationships of the varieties and lines, population structure of the different gene pools as well as linkage disequilibrium of closely linked markers. In part based on this study, many of the used markers are now the acknowledged standard for rapeseed variety fingerprinting


Cluster analysis of Brassica napus varieties and genebank material. Analyzed were 933 samples with 226 SSR markers

SNP markers

To date, a total of more than 15000 SNPs in over 2000 amplicons (genes) have been identified and collected in our marker database. For example, in spring oilseed rape, we have identified more than 1500 amplicons with SNPs. Within the analyzed winter oilseed rape lines, we have identified approximately 1200 amplicons with SNPs and in Brassica oleracea, more than 800 amplicons contain SNPs. Based on this SNP marker database, TraitGenetics is currently developing SNP markers through a variety of techniques ranging from individual Pyrosequencing assays to highly multiplexed marker sets with hundreds to thousands of SNPs markers.


Our genetic map of Brassica napus

With our set of proprietary markers derived from oilseed rape and Brassica oleracea, we have started to construct a genetic map of Brassica napus. So far this map consists of more than 1000 microsatellite markers and nearly 400 SNP markers. 

Integrated map of Brassica napus 
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